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1D NMR EXPERIMENTS |
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Proton Spectrum |
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DEPT |
This pulse technique
transfers magnetization from nuclei with large magnetic moment (1H)
to nuclei with small magnetic moment (13C, 15N) to
increase sensitivity |
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Difference NOE |
By irradiation of a signal
from one funcional group, the NOE difference spectrum
clearly shows the spatially adjacent groups. |
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Difference HOHAHA (Homonuclear HArtmann-HAhn) |
By irradiation of a signal
from one funcional group, the difference HOHAHA
spectrum shows spin transfer propagated along the carbon chain. |
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NOEDIFF |
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2D NMR EXPERIMENTS |
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HOMONUCLEAR J-RESOLVED
spin-echo |
The pulse used is the
simple Hahn spin-echo |
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The basic pulse sequence
is the Jeener experiment (COSY). This is a
magnetization transfer experiment |
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G-COSY |
Spin Echo correlated
spectroscopy |
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Double Quantum
Filtered-Correlated spectroscopy |
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TOCSY |
Total Correlation
Spectroscopy |
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A 2D experiment to
determine Nuclear Overhauser Enhancements |
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ROESY |
Rotating Frame Nuclear Overhauser Effect Spectroscopy |
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HETERONUCLEAR J-RESOLVED Gated-Decoupling
Spin-Flip Insesitive Nuclei Enhanced by
Polarization Transfer (INEPT) |
The observed nucleus (13C,15N,
31P) is J-Coupled to another species (1H) |
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HETERNUCLEAR SHIFT-CORRELATED HMQC |
Inverse heteronuclear multiple quantum correlation experiments |
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PHASE-CYCLED HMBC |
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Multiple bond connectivity
to obtain long range 1H/13C correlation by observation
of the more sensitive 1H nucleus |
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13C-13C DOUBLE QUANTUM INADEQUATE |
To establish13C-13C
J coupling and hence to determine the sequence of the 13C connectivities. |
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CP/MAS |
Combines cross polarization(1H) to a less sensitive nucleus
with Magic Angle Spinning at 54.7 degree to the magnetic field to enhance
sensitivity and reduce linewidth. |
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CRAMPS |
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Crasp |
Gradient Accelerated
Spectroscopy (Gradient-COSY) |
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DCP |
Double Cross Polarization |
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VACP |
Variable Amplitude Cross
Polarization |
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